Publications of S. Ruf

Wu, X.-X., Li, F., Zhu, C., Sun, S.-Y., Mu, W.-H., Ruf, S., Bock, R., Zhang, Y. & Zhou, F. Protocol for the purification of the plastid-encoded RNA polymerase from transplastomic tobacco plants. STAR Protocols 6, (2025).

Li, B., Armarego-Marriott, T., Kowalewska, Ł., Thiele, W., Erban, A., Ruf, S., Kopka, J., Schöttler, M. A. & Bock, R. Membrane protein provision controls prothylakoid biogenesis in tobacco etioplasts. The Plant Cell 36, 4862–4880 (2024).

Krämer, C., Boehm, C. R., Liu, J., Ting, M. K. Y., Hertle, A. P., Forner, J., Ruf, S., Schöttler, M. A., Zoschke, R. & Bock, R. Removal of the large inverted repeat from the plastid genome reveals gene dosage effects and leads to increased genome copy number. Nature Plants 10, 923–935 (2024).

Ghandour, R., Gao, Y., Laskowski, J., Barahimipour, R., Ruf, S., Bock, R. & Zoschke, R. Transgene insertion into the plastid genome alters expression of adjacent native chloroplast genes at the transcriptional and translational levels. Plant Biotechnology Journal 21, 711–725 (2023).

Strand, D., Karcher, D., Ruf, S., Schadach, A., Schöttler, M. A., Sandoval-Ibanez, O., Hall, D., Kramer, D. M. & Bock, R. Characterization of mutants deficient in N-terminal phosphorylation of the chloroplast ATP synthase subunit β. Plant Physiology 191, 1818–1835 (2023).

Wu, M., Zhang, Q., Dong, Y., Wang, Z., Zhan, W., Ke, Z., Li, S., He, L., Ruf, S., Bock, R. & Zhang, J. Transplastomic tomatoes expressing double-stranded RNA against a conserved gene are efficiently protected from multiple spider mites. New Phytologist 237, 1363–1373 (2023).

Chung, K. P., Gonzalez-Duran, E., Ruf, S., Endries, P. & Bock, R. Control of plastid inheritance by environmental and genetic factors. Nature Plants 9, 68–80 (2023).

Wu, M., Dong, Y., Zhang, Q., Li, S., Chang, L., Loiacono, F. V., Ruf, S., Zhang, I. & Bock, R. Efficient control of western flower thrips by plastid-mediated RNA interference. Proceedings of the National Academy of Sciences of the United States of America 119, (2022).

Agrawal, S., Karcher, D., Ruf, S., Erban, A., Hertle, A. P., Kopka, J. & Bock, R. Riboswitch-mediated inducible expression of an astaxanthin biosynthetic operon in plastids. Plant Physiology 118, 637–652 (2021).

Malinova, I., Zupok, A., Massouh, A., Schöttler, M. A., Meyer, E. H., Yaneva-Roder, L., Szymanski, W. G., Rößner, M., Ruf, S., Bock, R. & Greiner, S. Correction of Frameshift Mutations in the atpB Gene by Translational Recoding in Chloroplasts of Oenothera and Tobacco. The Plant Cell 33, 1682–1705 (2021).

Schulz, P., Piepenburg, K., Lintermann, R., Herde, M., Schöttler, M. A., Schmidt, L. K., Ruf, S., Kudla, J., Romeis, T. & Bock, R. Improving plant drought tolerance and growth under water limitation through combinatorial engineering of signaling networks. Plant Biotechnology Journal 19, 74–86 (2021).

Caroca, R., Howell, K. A., Malinova, I., Burgos, A., Tiller, N., Pellizzer, T., Annunziata, M. G., Hasse, C., Ruf, S., Karcher, D. & Bock, R. Knock-down of the plastid-encoded acetyl-CoA carboxylase gene uncovers functions in metabolism and development. Plant Physiology 185, 1091–1110 (2021).

Kössler, S., Armarego-Marriott, T., Tarkowska, D., Tureckova, V., Agrawal, S., Mi, J., Perez da Sousa, L., Schöttler, M. A., Schadach, A., Fröhlich, A., Bock, R., Al-Babili, S., Ruf, S., Sampathkumar, A. & Moreno, J. C. Lycopene β-cyclase expression influences plant physiology, development and metabolism in tobacco plants. Journal of Experimental Botany 72, 2544–2569 (2021).

Ruf, S., Kroop, X. & Bock, R. Chloroplast Transformation in Arabidopsis. Current Protocols in Plant Biology 1, (2021).

Fu, H.-Y., Ghandour, R., Ruf, S., Zoschke, R., Bock, R. & Schöttler, M. A. The availability of neither D2 nor CP43 limits the biogenesis of photosystem II in tobacco. Plant Physiology 185, 1111–1130 (2020).

Agrawal, S., Karcher, D., Ruf, S. & Bock, R. The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis. Plant Physiology 183, 263–276 (2020).

Vallarino, J. G., Kubiszewski-Jakubiak, S., Ruf, S., Rößner, M., Timm, S., Bauwe, H., Carrari, F., Rentsch, D., Bock, R., Sweetlove, L. J. & Fernie, A. R. Multi-gene metabolic engineering of tomato plants results in increased fruit yield up to 23. Scientific Reports 10, (2020).

Tabatabaei, I., Dal Bosco, C., Bednarska, M., Ruf, S., Meurer, J. & Bock, R. A highly efficient sulfadiazine selection system for the generation of transgenic plants and algae. Plant Biotechnology Journal 17, 638–649 (2019).

Ruf, S., Forner, J., Hasse, C., Kroop, X., Seeger, S., Schollbach, L., Schadach, A. & Bock, R. High-efficiency generation of fertile transplastomic Arabidopsis plants. Nature Plants 5, 282–289 (2019).

Legen, J., Ruf, S., Kroop, X., Wang, G., Barkan, A., Bock, R. & Schmitz‐Linneweber, C. Stabilization and translation of synthetic operon‐derived mRNAs in chloroplasts by sequences representing PPR protein‐binding sites. The Plant Journal 94, 8–21 (2018).