Publications of D. Karcher

Strand, D., Karcher, D., Ruf, S., Schadach, A., Schöttler, M. A., Sandoval-Ibanez, O., Hall, D., Kramer, D. M. & Bock, R. Characterization of mutants deficient in N-terminal phosphorylation of the chloroplast ATP synthase subunit β. Plant Physiology 191, 1818–1835 (2023).

Loiacono, F. V., Walther, D., Seeger, S., Thiele, W., Gerlach, I., Karcher, D., Schöttler, M. A., Zoschke, R. & Bock, R. Emergence of novel RNA editing sites by changes in the binding affinity of a conserved PPR protein. Molecular Biology and Evolution 39, (2022).

Tan, J., Forner, J., Karcher, D. & Bock, R. DNA base editing in nuclear and organellar genomes. Trends in Genetics 38, 1147–1169 (2022).

Hölscher, M., Forner, J., Calderone, S., Krämer, C., Taylor, Z., Loiacono, F. V., Agrawal, S., Karcher, D., Moratti, F. G., Kroop, X. & Bock, R. Expression strategies for the efficient synthesis of antimicrobial peptides in plastids. Nature Communications 13, (2022).

Frusciante, S., Demurtas, O. C., Sulli, M., Mini, P., Aprea, G., Diretto, G., Karcher, D., Bock, R. & Giuliano, G. Heterologous expression of Bixa orellana cleavage dioxygenase 4–3 drives crocin but not bixin biosynthesis. Plant Physiology 188, 1469–1482 (2021).

Ginsawaeng, O., Heise, C., Sangwan, R., Karcher, D., Hernández-Sánchez, I. E., Sampathkumar, A. & Zuther, E. Subcellular Localization of Seed-Expressed LEA_4 Proteins Reveals Liquid-Liquid Phase Separation for LEA9 and for LEA48 Homo- and LEA42-LEA48 Heterodimers. Biomolecules 11, (2021).

Agrawal, S., Karcher, D., Ruf, S., Erban, A., Hertle, A. P., Kopka, J. & Bock, R. Riboswitch-mediated inducible expression of an astaxanthin biosynthetic operon in plastids. Plant Physiology 118, 637–652 (2021).

Caroca, R., Howell, K. A., Malinova, I., Burgos, A., Tiller, N., Pellizzer, T., Annunziata, M. G., Hasse, C., Ruf, S., Karcher, D. & Bock, R. Knock-down of the plastid-encoded acetyl-CoA carboxylase gene uncovers functions in metabolism and development. Plant Physiology 185, 1091–1110 (2021).

Agrawal, S., Karcher, D., Ruf, S. & Bock, R. The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis. Plant Physiology 183, 263–276 (2020).

Tan, J., Zhang, F., Karcher, D. & Bock, R. Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nature Communications 11, (2020).

Xu, P., Chukhutsina, V. U., Nawrocki, W. J., Schansker, G., Bielczynski, L. W., Lu, Y., Karcher, D., Bock, R. & Croce, R. Photosynthesis without β-carotene. eLife 9, (2020).

Tan, J., Zhang, F., Karcher, D. & Bock, R. Engineering of high-precision base editors for site-specific single nucleotide replacement. Nature Communications 10, (2019).

Tan, J., Zhang, F., Karcher, D. & Bock, R. Author Correction: Engineering of high-precision base editors for site-specific single nucleotide replacement (Nature Communications, (2019), 10, 1, (439), 10.1038/s41467-018-08034-8). Nature Communications 10, (2019).

Moreno, J. C., Martinez-Jaime, S., Schwartzmann, J., Karcher, D., Tillich, M., Graf, A. & Bock, R. Temporal Proteomics of Inducible RNAi Lines of Clp Protease Subunits Identifies Putative Protease Substrates. Plant Physiology 176, 1485–1508 (2018).

van Oort, B., Roy, L. M., Xu, P., Lu, Y., Karcher, D., Bock, R. & Croce, R. Revisiting the Role of Xanthophylls in Nonphotochemical Quenching. The Journal of Physical Chemistry Letters 9, 346–352 (2018).

Liguori, N., Xu, P., van Stokkum, I. H. M., van Oort, B., Lu, Y., Karcher, D., Bock, R. & Croce, R. Different carotenoid conformations have distinct functions in light-harvesting regulation in plants. Nature Communications 8, (2017).

Lu, Y., Stegemann, S., Agrawal, S., Karcher, D., Ruf, S. & Bock, R. Horizontal Transfer of a Synthetic Metabolic Pathway between Plant Species. Current Biology 27, 3034–3041 (2017).

Moreno, J. C., Tiller, N., Diez, M., Karcher, D., Tillich, M., Schöttler, M. A. & Bock, R. Generation and characterization of a collection of knock-down lines for the chloroplast Clp protease complex in tobacco. Journal of Experimental Botany 68, 2199–2218 (2017).

Gnanasekaran, T., Karcher, D., Nielsen, A. Z., Martens, H. J., Ruf, S., Kroop, X., Olsen, C. E., Motawie, M. S., Pribil, M., Moller, B. L., Bock, R. & Jensen, P. E. Transfer of the cytochrome P450-dependent dhurrin pathway from Sorghum bicolor into Nicotiana tabacum chloroplasts for light-driven synthesis. Journal of Experimental Botany 67, 2495–2506 (2016).

Fuentes, P., Zhou, F., Erban, A., Karcher, D., Kopka, J. & Bock, R. A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop. eLife 5, (2016).