Publications of S. Ruf

Schöttler, M. A., Thiele, W., Belkius, K., Bergner, S. V., Flügel, C., Wittenberg, G., Agrawal, S., Stegemann, S., Ruf, S. & Bock, R. The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. Journal of Experimental Botany 68, 1137–1155 (2017).

Tabatabaei, I., Ruf, S. & Bock, R. A bifunctional aminoglycoside acetyltransferase/phosphotransferase conferring tobramycin resistance provides an efficient selectable marker for plastid transformation. Plant Molecular Biology 93, 269–281 (2017).

Gnanasekaran, T., Karcher, D., Nielsen, A. Z., Martens, H. J., Ruf, S., Kroop, X., Olsen, C. E., Motawie, M. S., Pribil, M., Moller, B. L., Bock, R. & Jensen, P. E. Transfer of the cytochrome P450-dependent dhurrin pathway from Sorghum bicolor into Nicotiana tabacum chloroplasts for light-driven synthesis. Journal of Experimental Botany 67, 2495–2506 (2016).

Gottschamel, J., Loessl, A., Ruf, S., Wang, Y., Skaugen, M., Bock, R. & Clarke, J. L. Production of dengue virus envelope protein domain III-based antigens in tobacco chloroplasts using inducible and constitutive expression systems. Plant Molecular Biology 91, 497–512 (2016).

Zhang, J., Khan, S. A., Hasse, C., Ruf, S., Heckel, D. G. & Bock, R. Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids. Science 347, 991–994 (2015).

Ehrnthaler, M., Scharff, L. B., Fleischmann, T. T., Hasse, C., Ruf, S. & Bock, R. Synthetic Lethality in the Tobacco Plastid Ribosome and Its Rescue at Elevated Growth Temperatures. The Plant Cell 26, 765–776 (2014).

Ruf, S. & Bock, R. Plastid transformation in tomato. Chloroplast Biotechnology: Methods and Protocols (2014). at <http://hdl.handle.net/11858/00-001M-0000-0014-1D9D-4>

Krech, K., Fu, H.-Y., Thiele, W., Ruf, S., Schöttler, M. A. & Bock, R. Reverse genetics in complex multigene operons by co-transformation of the plastid genome and its application to the open reading frame previously designated psbN. The Plant Journal 75, 1062–1074 (2013).

Caroca, R., Howell, K. A., Hasse, C., Ruf, S. & Bock, R. Design of chimeric expression elements that confer high-level gene activity in chromoplasts. Plant Journal 73, 368–379 (2013).

Shanmugabalaji, V., Besagni, C., Piller, L. E., Douet, V., Ruf, S., Bock, R. & Kessler, F. Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants. Plant Molecular Biology 81, 13–25 (2013).

Alkatib, S., Scharff, L. B., Rogalski, M., Fleischmann, T. T., Matthes, A., Seeger, S., Schöttler, M. A., Ruf, S. & Bock, R. The Contributions of Wobbling and Superwobbling to the Reading of the Genetic Code. PLoS Genetics 8, (2012).

Krech, K., Ruf, S., Masduki, F. F., Thiele, W., Bednarczyk, D., Albus, C. A., Tiller, N., Hasse, C., Schöttler, M. A. & Bock, R. The plastid genome-encoded Ycf4 protein functions as a non-essential assembly factor for photosystem I in higher plants. Plant Physiology 159, 579–591 (2012).

Zhang, J., Ruf, S., Hasse, C., Childs, L., Scharff, L. B. & Bock, R. Identification of cis-elements conferring high levels of gene expression in non-green plastids. Plant Journal 72, 115–128 (2012).

Elghabi, Z., Karcher, D., Zhou, F., Ruf, S. & Bock, R. Optimization of the expression of the HIV fusion inhibitor cyanovirin-N from the tobacco plastid genome. Plant Biotechnology Journal 9, 599–608 (2011).

Elghabi, Z., Ruf, S. & Bock, R. Biolistic co-transformation of the nuclear and plastid genomes. Plant Journal 67, 941–948 (2011).

Li, W., Ruf, S. & Bock, R. Chloramphenicol acetyltransferase as selectable marker for plastid transformation. Plant Molecular Biology 76, 443–451 (2011).

Albus, C. A., Ruf, S., Schöttler, M. A., Lein, W., Kehr, J. & Bock, R. Y3IP1, a Nucleus-Encoded Thylakoid Protein, Cooperates with the Plastid-Encoded Ycf3 Protein in Photosystem I Assembly of Tobacco and Arabidopsis. The Plant Cell 22, 2838–2855 (2010).

Ahlert, D., Stegemann, S., Kahlau, S., Ruf, S. & Bock, R. Insensitivity of chloroplast gene expression to DNA methylation. Molecular Genetics and Genomics 282, 17–24 (2009).

Fey, H., Piano, D., Horn, R., Fischer, D., Schmidt, M., Ruf, S., Schroeder, W. P., Bock, R. & Buechel, C. Isolation of highly active photosystem II core complexes with a His-tagged Cyt b559 subunit from transplastomic tobacco plants. Biochimica Et Biophysica Acta-Bioenergetics 1777, 1501–1509 (2008).

Ahmadabadi, M., Ruf, S. & Bock, R. A leaf-based regeneration and transformation system for maize (Zea mays L.). Transgenic Research 16, 437–448 (2007).