Publications of S. Ruf

Legen, J., Ruf, S., Kroop, X., Wang, G., Barkan, A., Bock, R. & Schmitz‐Linneweber, C. Stabilization and translation of synthetic operon‐derived mRNAs in chloroplasts by sequences representing PPR protein‐binding sites. The Plant Journal 94, 8–21 (2018).

Lu, Y., Stegemann, S., Agrawal, S., Karcher, D., Ruf, S. & Bock, R. Horizontal Transfer of a Synthetic Metabolic Pathway between Plant Species. Current Biology 27, 3034–3041 (2017).

Ruf, S. & Bock, R. Loopholes for smuggling DNA into pollen. Nature Plants 3, 918–919 (2017).

Scharff, L. B., Ehrnthaler, M., Janowski, M., Childs, L. H., Hasse, C., Gremmels, J., Ruf, S., Zoschke, R. & Bock, R. Shine-Dalgarno Sequences Play an Essential Role in the Translation of Plastid mRNAs in Tobacco. Plant Cell 29, 3085–3101 (2017).

Schöttler, M. A., Thiele, W., Belkius, K., Bergner, S. V., Flügel, C., Wittenberg, G., Agrawal, S., Stegemann, S., Ruf, S. & Bock, R. The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. Journal of Experimental Botany 68, 1137–1155 (2017).

Tabatabaei, I., Ruf, S. & Bock, R. A bifunctional aminoglycoside acetyltransferase/phosphotransferase conferring tobramycin resistance provides an efficient selectable marker for plastid transformation. Plant Molecular Biology 93, 269–281 (2017).

Gnanasekaran, T., Karcher, D., Nielsen, A. Z., Martens, H. J., Ruf, S., Kroop, X., Olsen, C. E., Motawie, M. S., Pribil, M., Moller, B. L., Bock, R. & Jensen, P. E. Transfer of the cytochrome P450-dependent dhurrin pathway from Sorghum bicolor into Nicotiana tabacum chloroplasts for light-driven synthesis. Journal of Experimental Botany 67, 2495–2506 (2016).

Gottschamel, J., Loessl, A., Ruf, S., Wang, Y., Skaugen, M., Bock, R. & Clarke, J. L. Production of dengue virus envelope protein domain III-based antigens in tobacco chloroplasts using inducible and constitutive expression systems. Plant Molecular Biology 91, 497–512 (2016).

Zhang, J., Khan, S. A., Hasse, C., Ruf, S., Heckel, D. G. & Bock, R. Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids. Science 347, 991–994 (2015).

Ehrnthaler, M., Scharff, L. B., Fleischmann, T. T., Hasse, C., Ruf, S. & Bock, R. Synthetic Lethality in the Tobacco Plastid Ribosome and Its Rescue at Elevated Growth Temperatures. The Plant Cell 26, 765–776 (2014).

Ruf, S. & Bock, R. Plastid transformation in tomato. Chloroplast Biotechnology: Methods and Protocols (2014). at <http://hdl.handle.net/11858/00-001M-0000-0014-1D9D-4>

Krech, K., Fu, H.-Y., Thiele, W., Ruf, S., Schöttler, M. A. & Bock, R. Reverse genetics in complex multigene operons by co-transformation of the plastid genome and its application to the open reading frame previously designated psbN. The Plant Journal 75, 1062–1074 (2013).

Caroca, R., Howell, K. A., Hasse, C., Ruf, S. & Bock, R. Design of chimeric expression elements that confer high-level gene activity in chromoplasts. Plant Journal 73, 368–379 (2013).

Shanmugabalaji, V., Besagni, C., Piller, L. E., Douet, V., Ruf, S., Bock, R. & Kessler, F. Dual targeting of a mature plastoglobulin/fibrillin fusion protein to chloroplast plastoglobules and thylakoids in transplastomic tobacco plants. Plant Molecular Biology 81, 13–25 (2013).

Alkatib, S., Scharff, L. B., Rogalski, M., Fleischmann, T. T., Matthes, A., Seeger, S., Schöttler, M. A., Ruf, S. & Bock, R. The Contributions of Wobbling and Superwobbling to the Reading of the Genetic Code. PLoS Genetics 8, (2012).

Krech, K., Ruf, S., Masduki, F. F., Thiele, W., Bednarczyk, D., Albus, C. A., Tiller, N., Hasse, C., Schöttler, M. A. & Bock, R. The plastid genome-encoded Ycf4 protein functions as a non-essential assembly factor for photosystem I in higher plants. Plant Physiology 159, 579–591 (2012).

Zhang, J., Ruf, S., Hasse, C., Childs, L., Scharff, L. B. & Bock, R. Identification of cis-elements conferring high levels of gene expression in non-green plastids. Plant Journal 72, 115–128 (2012).

Elghabi, Z., Karcher, D., Zhou, F., Ruf, S. & Bock, R. Optimization of the expression of the HIV fusion inhibitor cyanovirin-N from the tobacco plastid genome. Plant Biotechnology Journal 9, 599–608 (2011).

Elghabi, Z., Ruf, S. & Bock, R. Biolistic co-transformation of the nuclear and plastid genomes. Plant Journal 67, 941–948 (2011).

Li, W., Ruf, S. & Bock, R. Chloramphenicol acetyltransferase as selectable marker for plastid transformation. Plant Molecular Biology 76, 443–451 (2011).