Dr. Urszula Luzarowska

Dr. Urszula Luzarowska

wissenschftl. Mitarbeiter/-in
Abteilung 3 (Bock)
Photosyntheseregulation
PostDoc
+49 331 567 8303

Forschungsinteressen

I have obtained a PhD degree from Ben Gurion University in Israel. Working 4 years under the supervision of Dr. Yariv Brotman, I investigated natural variation in lipid metabolism of the plant Arabidopsis thaliana using mass spectrometry-based metabolomics techniques.

 

I developed my experience in group budget management and supervising undergraduate students.

 

I am currently working at the Max Planck Institute of Molecular Plant Physiology in Potsdam.

As a postdoctoral researcher in the group of Dr. Ute Armbruster, I investigate the effects of different natural and artificial light regimes on the regulation of several aspects of photosynthesis.

I aim to identify genetic determinants and associated metabolic signatures that regulate dynamic photosynthesis in different Arabidopsis accessions. To achieve this, I use both, forward and reverse genetic approaches, combined with metabolome and lipidome analyses.

Further, I plan to characterize newly found regulatory components, using spectroscopic, biochemical, molecular, and cell biological approaches.

In a complementary project, I study the effects of different natural and artificial light regimes on the metabolism of thylakoids, which is implicated in the regulation of several aspects of photosynthesis.

 

As a safety deputy of the group, I am responsible for the laboratory organization, especially people management for their safety during the COVID-19 pandemic.

 

As a laboratory manager, my responsibilities cover:

* Laboratory management (ordering, laboratory organization, risk assessments, external communications)

* People management (negotiation, motivation, introducing change, training, interviewing)

* Project management (planning, data delivery and analysis, presentation of results, progress reports)

* Managed laboratory staff, provided training for new employees 

Vita

EDUCATION

Ph.D. at Ben Gurion University of the Negev, Israel

2015 –2020

Thesis: Natural variation in 3-Ketoacyl-Coenzyme A Synthase 4: Glycerolipid Synthesis and Rearrangement in the Context of Starvation Response in Arabidopsis. Supervised by Dr. Yariv Brotman

MSc at Department of Biotechnology, University of Wroclaw, Poland

Specialization: Biotechnology of peptides and proteins

2013 - 2015

Thesis: Overexpression and purification of the protein HmuY homologs from the bacteria Prevotella intermedia and Tannerella forsythia. 
Supervised by Dr. Justyna Ciuraszkiewicz

BSc at Department of Biotechnology, University of Wroclaw, Poland

2009 - 2013

Theoretical thesis: Bacterial heme acquisition systems. 
Supervised by Prof. Dr. hab. Teresa Olczak


WORK AND RESEARCH EXPERIENCE

Postdoctoral researcher

Max Planck Institute of Molecular Plant Physiology, Germany

04/2020 - 

I joined research group of Dr. Ute Armbruster, I aim to identify and characterize regulatory mechanisms of photosynthesis. My particular focus is on the question of how photosynthesis is regulated to achieve efficiency in fluctuating light conditions.

Ph.D. 

Ben Gurion University of the Negev, Israel

2015 –2020

Thesis: Natural variation in 3-Ketoacyl-Coenzyme A Synthase 4: Glycerolipid Synthesis and Rearrangement in the Context of Starvation Response in Arabidopsis. Supervised by Dr. Yariv Brotman

Research project Erasmus

Max Planck Institute of Molecular Plant Physiology, Germany

08/2015 - 12/2015

I joined research group of Prof. Dr. Lothar Willmitzer as a trainee. I built expertise in molecular biology and different cloning strategies (CRISPR-PLANT©, GATEWAY®). My role was to design and perform experiments which led to generation of transgenic plants.

Research project Erasmus

Max Planck Institute of Molecular Plant Physiology, Germany

02/2015 - 06/2015

I joined research group of Dr. Vanessa Wahl as a trainee. My role was to culture Arabidopsis plants, genotype and phenotype plants using different molecular biology methods. I was responsible for preparation of reports and documentation of my laboratory work.

Training

Laboratory of Protein Biotechnology of the Faculty of Biotechnology at the University of Wrocław, Poland

07/2014 - 08/2014

I worked under supervision of Dr. Elżbieta Gocek. My role was to culture 
and handle mammalian cells.

Vocational training

HASCO-LEK Company in Wrocław by the project co-financed from European Union, Poland

08/2012 - 09/2012

I run independent laboratory work in the physicochemical quality control laboratory, I was involved in the planning of the experiments and discussion of the results with a supervisor

Internship

Laboratory of the Department of Biochemistry Faculty 
of Biotechnology

09/2010 - 08/2012

I joined the research group of Prof. Dr hab. Teresa Olczak as a trainee. I built expertise on biochemistry and proteomics. My role was to perform expression of recombinant proteins using prokaryotic system, protein purification (electrophoresis, affinity, hydrophobic and ion exchange chromatography, gel filtration) and preparation of laboratory equipment and reagents for further work. I developed good laboratory practice and communication skills.


PUBLICATION RECORD

* Toubiana, et al.; Scientific reports, 2020, “Correlation-based network analysis combined with machine learning techniques highlight the role of the GABA shunt in Brachypodium sylvaticum freezing tolerance”

* Treves, et al.; Nature Plants, 2020, “Multi-omics approach provides insights into extreme illumination stress response”

* Luzarowska, et al.; preprint in bioRxiv, 2020, “Hello darkness, my old friend: 3-Ketoacyl-Coenzyme A Synthase 4 is a branch point in the regulation of fatty acid rechanneling under carbon starvation in Arabidopsis thaliana

* Luzarowska, et al.; in preparation, “Identification of genes related to lipid metabolism in Arabidopsis thaliana in differential light and temperature stress conditions”

 

CONFERENCES

* Plants and People, Communicating Science – Connecting Worlds (2017), Potsdam, Germany

* Plant Stress Symposium on Oxidative and Abiotic Stress (2018), Potsdam, Germany

* 9th European Symposium on Plant Lipids (2019), Marseille, France


AWARDS

Incentive scholarship co-financed by European Union funds in semesters 1-6 (2010-2013)

 

LANGUAGES

* Polish - native speaker

* English – proficient

* German - elementary

 

COURSES AND WORKSHOPS

R workshop for Beginners. MPI of Molecular Plant Physiology, Potsdam, Germany (2019). Trainer: Dirk Walther.

Good Scientific Practice Course. MPI of Molecular Plant Physiology, Potsdam, Germany (2019) Trainers: Ina Talke, John Lunn and Friedrich Kragler 

Scientific Writing Workshop. MPI of Molecular Plant Physiology, Potsdam, Germany (2017). Trainer: Brian Cusack – Science Craft

Image processing for Scientists. MPI of Molecular Plant Physiology, Potsdam, Germany (2017). Trainer: Dr. Tina Koch – Mediomix

Presentation Skills course. MPI of Molecular Plant Physiology, Potsdam, Germany (2018). Trainer: Millie Baker 

Topics in Plant Genetics, Development and Evolution. MPI of Molecular Plant Physiology, Potsdam, Germany (2017). Trainers: Isabel Bäurle, Michael Lenhard, Stephan Greiner, Stefanie Hartmann, Roosa Laitinen, Aleksandra Skirycz, Salma Balazadeh, Ralph Bock, Markus Grebe

 

TECHNICAL SKILLS

* Metabolite profiling using UPLC-MS and GC-MS with special focus on lipophilic compounds

* Analysis and processing of raw MS data using Expressionist Refiner MS® (GeneData) and Xcalibur (Thermo-Fisher)

* Extracting knowledge from big data sets, including data visualization using R language

* Genetic mapping (GWAS)

* Sample preparation (DNA, RNA, and proteins) and classic molecular biology techniques (qRT-PCR, WB, SDS-PAGE, cloning, CRISPR-PLANT©)

* Execution of enzyme activity assays

 

GENERAL SKILLS

PROJECT MANAGEMENT, MENTORING EXPERIENCE, TEAMWORK, EXCELLENT COMMUNICATION SKILLS

Management of research project, which will result in submitting a manuscript in an international journal (first author manuscript is under revision). Mentoring an MSc student project, which will result in submitting a manuscript to an international journal (first co-author manuscript in preparation).

Good teamwork and collaboration skills (2 manuscripts in press and several manuscripts to be submitted). Supervision of seven undergraduate students leading to their independence in the laboratory. Participation in international conferences by sharing ideas and communicating results.

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