Dr. Maria Grazia Annunziatawissenschftl. Mitarbeiter/-in
NAME: Maria Grazia Annunziata
DATE OF BIRTH: 5th February 1983
WORK ADDRESS: Max Planck Institute of Molecular Plant Physiology,
Am Mühlenberg 1,
14476 Potsdam (OT) Golm,
TEL: +49 331 567-8169
FAX: +49 331 567 8408
Brief description of myself:
I am Maria Grazia Annunziata, a postdoctoral fellow in the group of Prof. Dr Mark Stitt at the Max Planck Institute of Molecular Plant Physiology.
I have a background in primary and secondary metabolite profiling and abiotic stress responses in model and crop plants. The main focus of my current work is investigating the effects of different natural and artificial light regimes on carbon metabolism (including transitory starch synthesis and degradation) and the circadian clock, which is implicated in diurnal regulation of several aspects of plant central metabolism. In a complementary project, I am studying the possible role of trehalose 6-phosphate (Tre6P) in the regulation of stomatal conductance in Arabidopsis thaliana.
I enjoy engaging with my colleagues in the scientific community. For example, I am currently serving as one of the elected representatives of the postdoctoral community within the institute, which includes organizing training courses, representing junior researchers at institute management meetings. Over 40 nationalities are represented in our institute and I have participated in several cultural awareness courses and in open days for the general public, so with more than 10 years of experience of working in plant biology, an international perspective and a keen interest in scientific writing, I am used to communicating with people from diverse backgrounds and levels of scientific education. I take an active role in drafting manuscripts, and helping students with editing and proofreading of their interim reports and PhD theses, which has given me experience and a broad perspective on different types of scientific writing. I actively scan the plant science literature and enjoy discussing new papers with my colleagues. In the period 2018-2019 I had the great opportunity to be one of the Associate Features Editor of Plant Physiology. Serving the scientific community in this role increased even more my passion for plant science.
2001-2005 - Laurea Triennale in Scienze Biologiche (equivalent to Bachelors in Biology), Seconda Universitá degli Studi di Napoli, Caserta, Italy
2005-2008 - Laurea Specialistica in Biologia (equivalent to Masters in Biology) (graduated with Distinction), Seconda Universitá degli Studi di Napoli, Caserta, Italy
2008-2011 - Ph.D. Seconda Universitá di Napoli, Caserta, Italy
Thesis: “Analysis of metabolites and identification of mutants with altered starch degradation in Brassicaceae”; Supervisor: Prof. Amodio Fuggi
2012 to present – Post-doctoral fellow, System Regulation Group, Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany. Supervisor: Prof. Dr Mark Stitt.
OTHER PROFESSIONAL ACTIVITIES
March 2018 to December 2019 - Plant Physiology Associate Features Editor
Active Reviewer of Agronomy, Journal of Experimental Botany and PlosOne
Since February 2020 - IATA (International Air Transport Association) Shipper for the MPI-MP. I am one of the certified MPI-MP employees responsible for shipping dangerous goods via air transport.
MPI-MP PostDoc Representative – May 2014 – at present
Beside representing junior researchers at institute management meetings, I have been active in organizing transferable skills courses for my peers at the MPI-MP (Scientific Writing, Research Funding and Career Development, Team and Leadership Skills, Grant Writing, How to start your own lab). Moreover, I organized career talks (Ralf Neumann – Editor of “Laborjournal” and “Lab Times”; Mary Williams - Features Editor of “The Plant Cell”; Christopher Surridge - Editor-in-chief of “Nature Plants”) and “Foresight Talks” in which renowned plant scientists share their ideas about what science will look like in the future. The scientists I invited were female professors to provide role-models for young female scientists (Alison Smith - Professor at the John Innes Centre, Norwich, UK; Ottoline Leyser - Professor at the Sainsbury Laboratory, Cambridge, UK).
Member of the MPI Minerva-FemmeNet mentoring programme – since November 2014
Enzymatic analysis of metabolites using spectrophotometric and fluorimetric techniques
LC-MS/MS – analysis of phosphorylated sugars and organic acid determination
HPLC and Ion-Chromatography (Dionex) – measurement of amino acids, sugars, anions and cations (organic and inorganic), glucosinolates
PCR – genotyping
Real-Time qRT-PCR – transcript analysis
Plant growth (Arabidopsis thaliana, Triticum Durum)
Use of Microsoft Office (Word, Excel, PowerPoint), SigmaPlot, LIMS database, XLSTAT (Statistical Software for Excel), Minitab
Italian: mother tongue
English: spoken – excellent; written – excellent
IELTS (British Council) Certificate – Overall Band Score: 7.5/9 (Very Good User; February 2018)
German: spoken – good; written – good
TELC Test Certificate - hören/lesen:45/45; schreiben:20/20; sprechen:20/20 (18.09.2013)
Papers in refereed journals
Annunziata MG. (2019) NTRC: a key regulatory hub in carbon metabolism and redox balance in developing tomato fruits. Plant Physiology. News and Views, in press.
Annunziata MG. (2019) Enhancing wheat rubisco activase thermostability by mutagenesis of conserved residues from heat-adapted species. Plant Physiology News and Views, 181: 3.
Annunziata MG. (2019) What is lignin made of? New components discovered! Plant Physiology News and Views, 180:1255.
Annunziata MG. (2019) Plant leaf apoplast: an easy method to estimate its hydration state and contents. Plant Physiology News and Views, 180:688.
Annunziata MG, Ciarmiello LF, Woodrow P, Dell'Aversana E, Carillo P (2019). Spatial and temporal profile of glycine betaine accumulation in plants under abiotic stresses. Frontiers in Plant Science, doi: 10.3389/fpls.2019.00230.
Moraes TA, Mengin V, Annunziata MG, Encke B, Krohn N, Höhne M, Stitt M (2019). Response of the circadian clock and diel starch turnover to one day of low light or low CO2. Plant Physiology 179(4):1457-1478.
Ciarmiello LF, Di Maro A, Woodrow P, Annunziata MG, Kafantaris I, Mirto A, Iannuzzi F, Fuggi A, Carillo P (2018). Unveiling the enigmatic structure of TdCMO transcripts in durum wheat. Agronomy 8, 270; doi:10.3390/agronomy8110270.
Annunziata MG, Apelt F, Carillo P, Krause, Feil R, Koehl K, Lunn JE, and Stitt M (2018). Response of Arabidopsis primary metabolism and circadian clock to low night temperature in a natural light environment. Journal of Experimental Botany, 69(20):4881-4895.
Annunziata MG. (2018) The long and the short of it: GA 2-oxidaseA9 regulates plant height in wheat. Plant Physiology News and Views 177(1):3-4.
Fichtner F, Barbier FF, Feil R,Watanabe M, Annunziata MG, Chabikwa TG, Höfgen R, Stitt M, Beveridge CA, Lunn JE. (2017) Trehalose 6-phosphate is involved in triggering axillary bud outgrowth in garden pea (Pisum sativum L.). The Plant Journal, doi: 10.1111/tpj.13705.
Fusari CM, Kooke R, Lauxmann MA, Annunziata MG, Encke B, Hoehne M, Krohn N, Becker FFM, Schlereth A, Sulpice R, Stitt M, Keurentjes JJB. (2017) Genome-wide association mapping reveals that specific and pleiotropic regulatory mechanisms fine-tune central metabolism and growth in Arabidopsis. The Plant Cell, doi: 10.1105/tpc.17.00232.
Annunziata MG, Apelt F, Carillo P, Krause U, Feil R, Mengin V, Lauxmann MA, Köhl K, Nikoloski Z, Stitt M, Lunn JE. (2017). Getting back to nature: a reality check for experiments in controlled environments. Journal of Experimental Botany 68, 4463–4477.
Apelt F, Breuer D, Olas JJ, Annunziata MG, Flis A, Nikoloski Z, Kragler F, Stitt M. (2017) Circadian, carbon, and light control of expansion growth and leaf movement. Plant Physiology 174, 1949-1968.
Annunziata MG, Ciarmiello LF, Woodrow P, Maximova E, Fuggi A, Carillo P (2017). Durum wheat roots adapt to salinity remodeling the cellular content of nitrogen metabolites and sucrose. Frontiers in Plant Science, 7, 1-16.
Woodrow P, Ciarmiello LF, Annunziata MG, Pacifico S, Iannuzzi F, Mirto A, D´Amelia L, Dell´Aversana E, Piccolella S, Fuggi A and Carillo P. (2017). Durum wheat seedling responses to simultaneous high light and salinity involve a fine reconfiguration of amino acids and carbohydrate metabolism. Physiologia Plantarum 159, 290-312.
Lauxmann MA, Annunziata MG, Brunoud G, Wahl V, Koczut A, Burgos A, Olas JJ, Maximova E, Abel C, Schlereth A, Soja AM, Bläsing OE, Lunn JE, Vernoux T, Stitt M (2016). Reproductive failure in Arabidopsis thaliana under transient carbohydrate limitation: flowers and very young siliques are jettisoned and the meristem is maintained to allow successful resumption of reproductive growth. Plant Cell and Environment, 39: 745-67.
Ragel P, Streb S, Feil R, Sahrawy M, Annunziata MG, Lunn JE, Zeeman S, Mérida Á (2013). Loss of starch granule initiation has a deleterious effect on the growth of Arabidopsis plants due to an accumulation of ADP-glucose. Plant Physiology, 163:75-85.
Annunziata MG, Carillo P, Fuggi A, Troccoli A, Woodrow P (2013). Metabolic profiling of cauliflower under traditional and reduced tillage systems. Australian Journal of Crop Science, 7:1317-1323.
Annunziata MG, Attico A, Woodrow P, Oliva MA, Fuggi A, Carillo P (2012). An improved fluorimetric HPLC method for quantifying tocopherols in Brassica rapa L. subsp. sylvestris after harvest. Journal of Food Composition and Analysis, 27:145-150.
Woodrow P, Ciarmiello LF, Fantaccione S, Annunziata MG, Pontecorvo G, Carillo P (2012). Ty1-copia group retrotransposons and the evolution of retroelements in several angiosperm plants: evidence of horizontal transmission. Bioinformation, vol. 8, p. 267-271.
Woodrow P, Fuggi A, Pontecorvo G, Kafantaris I, Annunziata MG, Massaro G, Carillo P (2012). cDNA cloning and differential expression patterns of ascorbate peroxidase during post-harvest in Brassica rapa L.. Molecular Biology Reports, 39(8):7843-53.
Carillo P, Parisi D, Woodrow P, Pontecorvo G, Massaro G, Annunziata MG, Fuggi A, Sulpice R (2011). Salt induced accumulation of glycine betaine is inhibited by high light in durum wheat. Functional Plant Biology 38,139-150.
Annunziata MG and Lunn JE (2020) Regulation of assimilatory starch metabolism by cellular carbohydrate status. In: Enzymology of Complex Glycans (eds Martin Steup, Felix Nitschke). CRC Press, Taylor & Francis Ltd., London. ISBN13 9781138505209.
Woodrow P, Pontecorvo G, Ciarmiello LF, Annunziata MG, Fuggi A, Carillo P (2012). Transcription factors and Genes in Abiotic stress. In: Venkateswarlu B, Shanker AK, Shanker C, Maheswari M. Crop stress management: perspective and strategies. p. 317-357, Springer Netherlands, ISBN: 978-94-007-2219-4.
Carillo P, Annunziata MG, Pontecorvo G, Fuggi A, Woodrow P (2011). Salinity stress and salt tolerance. In: Arun Shanker. Abiotic stress - mechanisms and adaptations. vol. 1, p. 21-38, ISBN: 978-953-307-394-1.
Presentations at meetings
Feil R, Annunziata MG, Stitt M, Lunn JE. Measuring Trehalose 6-phosphate (Tre6P) in different plant materials – the technical challenge of working at the femtomole scale. XXII International Mass Spectrometry Conference, Florence, Italy (26th-31st August 2018) (poster presentation)
Annunziata MG, Apelt F, Lauxmann MA, Krause U, Feil R, Koehl K, Stitt M, Lunn JE.
Getting back to nature: a reality check for experiments in controlled environments. 11th International Congress of Plant Molecular Biology, Iguazú Falls, Brasil (25-30th October
2015) (poster presentation)
Annunziata MG, Maximova E, Stitt M, Hartmann J, Lunn JE. Morphology of leaf starch granules of Arabidopsis thaliana wild type and mutants. 18th International Microscopy Congress, Prague, Czech Republic (7th -12th September 2014) (poster presentation)
Annunziata MG, Koehl K, Carillo P, Maximova E, Feil R, Krause U, Lunn JE, Stitt M. Investigating the regulation of starch metabolism using a novel Arabidopsis starch excess mutant. Society of Experimental Biology Annual Meeting, Manchester, UK (1st-4th July 2014) (poster presentation)
Annunziata MG, Koehl K, Carillo P, Feil R, Krause U, Lunn JE, Stitt M. Screening of Arabidopsis thaliana T-DNA insertion lines for novel mutants with defects in starch metabolism. FESPB Congress, Freiburg, DE (29th July-3rd August 2012) (poster presentation)
COURSES AND WORKSHOPS
R workshop for Beginners. MPI of Molecular Plant Physiology, Potsdam, Germany (April-May 2017 - 6 sessions). Trainer: Dirk Walther.
Negotiation basics: Common pitfalls and success strategies for women. MPI for Human Development, Berlin, Germany (28th October 2016) Trainer: Prof. Dr. Brooke A. Shaughnessy
Fundamentals of mass spectrometry. XXII International Mass Spectrometry Conference, Toronto, Canada (20th –21st August 2016). Trainers: Jürgen H. Gross & O. David Sparkman
Grant Writing Workshop. Harnack House – Conference Venue of the Max Planck Society, Berlin, Germany (28th – 29th April 2016). Trainer: Babette Regierer.
Master your scientific project successfully. Harnack House – Conference Venue of the Max Planck Society, Berlin, Germany (14th – 15th October 2015). Trainer: Barbara Wagner
PerForming Teams - Executing leadership in Science: how to manage a successful team.
Harnack House – Conference Venue of the Max Planck Society, Berlin, Germany (29th – 30th September 2015). Trainers: Schiller and Mertens.
Scientific Writing Workshop. MPI of Molecular Plant Physiology, Potsdam, Germany (15th- 16th July 2015). Trainers: Justin Mullins and Mark Buchanan -WAS Write About Science
Research Founding and Career Development. Fritz Haber Institute of the Max Planck Society, Berlin, Germany (9th - 10th December 2014). Trainer: Dr. Beate Scholz - Scholz – consulting training coaching.
Job Hunting MPI of Molecular Plant Physiology, Potsdam, Germany (17th of January 2013). Trainer: Ralf Petri
An Introduction to Intercultural Communication for Doctoral Students: Developing Key Problem-Solving Skills for the International Workplace. MPI of Molecular Plant Physiology, Potsdam, Germany (15th -16th October 2012). Trainer: Alexia Petersen
How to Lead a Lab. MPI of Molecular Plant Physiology, Potsdam, Germany (6th of September 2012). Trainer: Michael Schroda
Plant cultivation, experimental design and statistics. MPI of Molecular Plant Physiology, Potsdam, Germany (16th -27th April 2012). Trainer: Karin Köhl.
EXPERIENCE IN SUPERVISING UNDERGRADUATE STUDENTS
February 2020 – at present. Supervisor of Sarah Isabel Richard. Master student from the University of Potsdam hosted at the Max Planck Institute of Molecular Plant Physiology (MPI-MP).
March 2018 – March 2020. Supervisor, during their various visits at the MPI-MP, of Emilia Dell’Aversana and Giovanna Marta Fusco, PhDs of Universita’ della Campania “Luigi Vanvitelli” within the DAAD-MIUR Project No. 32983: the Italy-Germany Joint Mobility Program “Salinity tolerance and nitrogen use efficiency in durum wheat”.
September 2018 - February 2019. Supervisor of Romane Lahaye, ERASMUS student at the MPI-MP.
August 2018. Supervisor of Luisa D’Amelia, Visiting Researcher Student of Universita’ della Campania “Luigi Vanvitelli”.
August 2017 – September 2017. Supervisor of Emilia Dell’Aversana, Master student of Universita’ della Campania “Luigi Vanvitelli”.
March 2017 – June 2017. Supervisor of Jagoda Szwedyk, student-helper at the MPI-MP.
February 2016 – March 2016. Supervisor of Bryan Nowack, student-helper at the MPI-MP.
October 2015 - December 2015. Supervisor of Jana Verbancic, IAESTE Master-student at the MPI-MP.
July 2015 – September 2015. Supervisor of Rafal Zdrzalek, ERASMUS PLUS student at the MPI-MP.
July 2015 – September 2015. Supervisor of Katarzyna Czernik, ERASMUS PLUS student at the MPI-MP.
December 2013 - June 2014. Co-supervisor of Marina Salles, IAESTE student at the MPI-MP.
July 2012 - September 2012. Supervisor of Michał Śmiga student-helper at the MPI-MP.