Dr Monika Chodasiewicz (Kosmacz)
My work is focused on cAMP as small signaling molecule that acts as a secondary messenger in animal cells and it is known to be involved in regulation of many important biological processes including primary metabolism, cell cycle, response to stress and hormonal homeostasis. Despite the fact that cAMP-dependent signaling cascades are well understood in animals, little is known about its potential function in plants. Although the presence of cAMP was recently confirmed in plant cells its protein interactors, with the exception of the family of the CNGC ion channels, are still nonidentified.
In order to identify potential cAMP – protein interactions in plants, we decided to apply a range of new approaches, initially developed for mode of action research in the pharmacology field. Specifically, we adapted Drug Affinity Responsive Target Stability (DARTS) and Thermal Proteome Profiling (TPP) assays to plant lysates. Moreover, to complement DARTS and TPP, we also performed classical affinity purification using cAMP agarose beads. Starting with native lysates prepared from Arabidopsis cell cultures and using state of the art proteomic approach, we identified several proteins as candidates for cAMP interactors. At present, biophysical studies are performed to determine the binding affinity of these proteins to cAMP as a further validation of their role as cAMP binding proteins.
With our studies we hope to unravel the mystery of cAMP signaling in plants.
1.09.2010 - 28.05.2014 PhD in Molecular Plant Physiology at Max Plank Institute
o Dissertation: Characterization of the oxygen sensing mechanism in plants. under supervision of Prof. Dr. Joost van Dongen
1.09.2005 - 31.08.2010 Diploma in Biotechnology at University of Szczecin, Poland.
o Thesis (Master of Science equivalent): Studies on the dynamic of the karyokinetic spindle in pollen mother cells of Tritisecale crosses under supervision of Prof. Stanisława Rogalska
01.09.2002 – 31.08.2005 Secondary School nr X, ul. Orawska 3 in Szczecin, Poland
WORK AND RESEARCH EXPERIENCE
1.06.2015 – now Postdoc position at Prof. Dr. Lothar Willmitzer group at the Max-Planck-Institute for Molecular Plant Physiology
29.05.2014 – 29.05.2015 Postdoc position at Dr. Fritz Kragler group at the Max-Planck-Institute for Molecular Plant Physiology
01.09.2010 – 28.05.2014 PhD at the Max-Planck-Institute for Molecular Plant Physiology
01. 09. 2009 – 28. 02. 2010 Internship at the Max-Planck-Institute for Molecular Plant Physiology, Potsdam-Golm, Germany
01. 08. 2009 – 31. 08. 2009 Internship at University of Szczecin, Department of cell Biology
01. 07. 2009 – 30. 07. 2010 Internship at Forensic Medicine
1. Bui L.T, Giuntoli B., Kosmacz M., Parlanti S., Licausi F. (2015) “Constitutively expressed ERF-VII transcription factors redundantly activate the core anaerobic response in Arabidopsis thaliana’’ Plant Science doi: 10.1016/j.plantsci.2015.03.008
2. Kosmacz M., Parlanti S., Schwarzlander M., Kragler F., Licausi F., van Dongen J.T. (2014) “Oxygen Sensing in Plants - Concentration Dependent and Time-Resolved Characterization of RAP2.12 Stability and Localization” Plant Cell and Environment doi: 10.1111/pce.12493
3. Giuntoli B., Lee C.S., Licausi F., Kosmacz M., Oosumi T., van Dongen J.T., Bailey-Serres J., Perata P. (2014) “A trihelix DNA binding protein counterbalances hypoxia-responsive transcriptional activation in Arabidopsis” Plos Biology doi: 10.1371/journal.pbio.1001950
4. Weits D., Giuntoli B., Kosmacz M., Parlanti S., Hubberten H-M., Hoefgen R., Perata P., van Dongen J.T. and Licausi F. (2014) “Plant cysteine oxidases control the oxygen-dependent branch of the N-end rule pathway” Nature communications doi:10.1038/ncomms4425
5. Kosmacz M., and Weits D., 2014 “Oxygen perception in plants” van Dongen J.T., and Licausi F., (eds.) Low oxygen stress in plants. Springer, Heidelberg.
6. Licausi F., Kosmacz M., Weits D., Giuntoli B., Giorgi F.M., Voesenek L.A.C.J., Perata P., and van Dongen J.T. (2011) “Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization” Nature 479, 419-422
7. Paparelli E., Gonzali S., Parlanti S., Novi G., Giorgi F. M., Licausi F., Kosmacz M., Feil R. Lunn J.E., Brust H., van Dongen J.T., Steup M., and Perata P. (2012) “Misexpression of a Chloroplast Aspartyl Protease Leads to Severe Growth Defects and Alters Carbohydrate Metabolism in Arabidopsis” Plant Physiology 160: 1237-1250
8. Veyel D., Kierwszniowska S., Kosmacz M., Sokolowska E.M., Michaelis A., Luzarowski M., Szlachetko J., Willmitzer L., Skirycz A. (2017) ‘’System-wide detection of protein-small molecule complexes suggests extensive metabolite regulation in plants’’ Scientific Reports doi:10.1038/srep42387