Nuclear calcium signalling is known to be essential in legumes to promote associations with nitrogen fixing bacteria and phosphate delivering arbuscular mycorrhizal fungi. Legumes are among the world’s most important agricultural food crops that are beneficial to billions of farmers and consumers worldwide and provide an essential aspect of natural soil enrichment of organic nitrogen compounds. The mechanisms of plant nuclear calcium signalling were poorly understood. Using a wide range of approaches, we have discovered a number of ion channels located at the nuclear envelope that are responsible for symbiotic nuclear calcium release. Amon g them, we defined the first plant nuclear-associated calcium channels encoded by cyclic nucleotide gated channels (CNGC15s). The CNCG15s sit at the nuclear envelope in a complex with a potassium permeable channel (DMI1), also required for the generation of the symbiotic nuclear calcium signals. Interestingly, CNGC15s and DMI1 are conserved across all land plants, including non-symbiotic species, strongly suggesting that they have other functions during plant development. The function of this nuclear calcium channel complex in and outside root legume symbioses will be discussed.
2018 Kelner A, Leitao N, Chabaud M, Charpentier M* and Carvalho-Niebel F*. Dual color sensors for simultaneous analysis of calcium signal dynamics in the nuclear and cytoplasmic compartments of plant cells. Frontiers in Plant Science. in press
2018 Cooke A, Charpentier M*. Encoding nuclear ca lcium oscillations in root symbioses. In: de Bruijn FJ. (Ed) The Model Legume Medicago truncatula. Wiley Blackwell Publishers, Chichester, UK. in press.
2017 Thorpe SD, Charpentier M*. Highlight on the dynamic organization of the nucleus. Nucleus. 8(1):2-10.
2016 Charpentier M*, Sun J, Vas MartinezT, et al., Nuclear-localised cyclic nucleotide gated channels mediate symbiotic calcium oscillations. Science. 352(6289):1102-05.
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