Guest Group

Dr. Yariv Brotmann
Group Leader
Phone:+49 331 567-8228

Ben-Gurion University of Negev

Department Willmitzer (Guest Group)

Genetics of Metabolic Traits

Research in the group of Dr. Yariv Brotman focuses on the use of quantitative genetic approaches for the identification of genes that are involved in plant metabolism, and on further functional characterization of those genes by application of different molecular biology techniques and transgenic plant approaches. Special focus is given to genes that are taking part in lipid metabolism, but also genes that are taking parts in primary and secondary metabolism are investigated. The group is also integrating knowledge from classic lipid biochemistry with lipidomics in order to refine the knowledge on lipid biochemical pathways.
<strong>Overview of the <em>Arabidopsis</em> genetic map resulted from the GWAS</strong>. Circles refer to co-localization of GWA mapping hits on chromosomes with –log<sub>10 </sub>(<em>P</em>-value) of SNP-lipid associations which were significant with FDR ≤ 0.05. Dotted lines indicate genomic positions with association signals of multiple lipids. Candidate genes, harbor within the loci, with known relationships to lipid metabolism or that show sequences homology to lipid metabolism genes are indicated. Zoom Image
Overview of the Arabidopsis genetic map resulted from the GWAS. Circles refer to co-localization of GWA mapping hits on chromosomes with –log10 (P-value) of SNP-lipid associations which were significant with FDR ≤ 0.05. Dotted lines indicate genomic positions with association signals of multiple lipids. Candidate genes, harbor within the loci, with known relationships to lipid metabolism or that show sequences homology to lipid metabolism genes are indicated. [less]

Projects:

<p>In recent years, complete genome sequences for <em>Arabidopsis thaliana</em>, maize and rice have been completed and high-density single-nucleotide-polymorphism (SNP) molecular markers have become available for a large number of accessions (natural variation) of these plants. These allow the conducting of genome-wide association studies (GWAS) for the identification of the genetic regulation of different phenotypic traits.</p>

Quantitative Genetics

In recent years, complete genome sequences for Arabidopsis thaliana, maize and rice have been completed and high-density single-nucleotide-polymorphism (SNP) molecular markers have become available for a large number of accessions (natural variation) of these plants. These allow the conducting of genome-wide association studies (GWAS) for the identification of the genetic regulation of different phenotypic traits.

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Functional characterization of MYB transcription factors in Arabidopsis

In plants, MYB transcription factors (TF) play a key role in different aspects of plant development, secondary metabolism, hormone signal transduction, disease resistance and abiotic stress tolerance [more]

Systems biology approach to understand lipid metabolism in high oil maize lines

Together with the groups of Prof. Zoran Nikoloski (University of Potsdam, Germany), Prof. Yan Jianbing and Prof. Weiwei Wen (Huazhong Agricultural University, Wuhan, China), we are employing an approach that integrates the time-resolved  metabolomics and transcriptomics joint analysis with mQTL genetic mapping of maize to elucidate the association between genes and acyl-lipids. [more]

Plant lipidomics: Refining pathway knowledge with a new technology

The large majority the knowledge we have on plant metabolism was generated before liquid chromatography-mass spectrometry-based (LC-MS) lipidomics was available. This translates into the fact that we have a new technique which results can confirm or not reviously generated hypotheses. Interestingly, some of our observations on lipid composition in the plant model Arabidopsis thaliana showed discrepancies with generally accepted ideas. we reported the existence of new compounds belonging to ER-synthesized classes of lipids containing 16:3 or its precursor 16:21, fatty acids that are typical of the chloroplast and deem to be absent in lipids with ER origin. The challenge imposed by these compounds was big: how to explain the existence of compounds that are not supposed to exist, and more importantly, how to reconcile it with more than 30 years of studies in lipid biochemistry. [more]

Group members:

Yariv Brotman, PhD (Guest group leader, BGU)

Asdrubal Burgos, PhD (Guest Scientist, BGU): Systems biology of lipid metabolism

Isabel Orf, PhD (Guest Scientist, BGU): GWAS of lipids in rice

Si Wu (Doctoral student): GWAS of primary and secondary metabolites in Arabidopsis

Ke Xu (Doctoral student): MYB transcription factors

Francisco de Abreu e Lima (Doctoral student): Lipid metabolism in high oil maize lines

Urszula Luzarowska (Doctoral student, BGU): Systems biology of lipid metabolism, GWAS of lipids in Arabidopsis

Anastasiya Kuhalskaya (Doctoral student, BGU): GWAS of lipids in tomato

 

 
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